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KMID : 0358419970400010154
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Korean Journal of Obstetrics and Gynecology
1997 Volume.40 No. 1 p.154 ~ p.160
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Inhibition of Cell Growth and Suppression of c-myc Gene Expression by Transforming Growth Factor-β1 in Cervical Cell Lines
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김진우
김미란/김재훈/김태응/박태철/이헌영/김승조/남궁성은
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Abstract
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Transforming growth factor-β1(TGF-β1 ) is known to be a potent growth inhibitor for many cell types, including most epithelial cells. In skin keratinocytes, TGF-β1 has been shown to inhibit growth and to rapidly reduce c-myc expression.
However,
the
molecular mechanism of TGF-β1 action on cell growth of cervical carcinoma has not yet been elucidated. We thus assessed the effect of TGF-β1 on the growth of cervical carcinoma cell lines. Two cervical squamous carcinoma cell lines were
incubated
with
varying concentration of TGF-β1 , and growth inhibition was evaluated with tetrazolium-based colorimetric assay. After culturing in concentrations of 0.1∼10ng/ml in both cell lines. Northern blot analysis revealed c-myc mRNA expression was
suppressed
by 10ng/ml of TGF-β1 following 6-hour of treatment in both cell lines. These results suggest that TGF-β1 inhibits the growth of cervical carcinoma cells by suppressing c-myc oncogene expression.
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